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September 13, 2024

Preparation of B cells for scRNAseq v1

Nicholas A. Pease

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Key Points

The protocol aims to standardize the preparation of activated b cell suspensions to support microfluidic droplet-based partitioning workflows.
Sample hashtagging is incorporated to allow multiplexing of distinct experimental samples for chromium single cell gene expression profiling.
Protocol optimization supports downstream 10x genomics platforms, which may enable cost-effective and reproducible scrnaseq experiments.

Implication

Protocol demonstrates preparation and hashtagging in activated B cell cultures, highlighting streamlined single-cell gene expression profiling.

Abstract

This protocol details activated B cell preparation and sample hashtagging for Chromium Single Cell Gene Expression (10x Genomics).

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Cite This Study

Nicholas A. Pease (2024) studied this question.

synapsesocial.com/papers/68e58a50b6db643587525ef5 https://doi.org/10.17504/protocols.io.n92ld8e1nv5b/v1

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