Abstract Receptors for activated C kinase (RACKs) have been shown to coordinate PKC-mediated hypertrophic signaling in the mouse. However, rare information is available on its participation in embryonic gene expression. This study investigated the involvement of RACK1 in the expression of embryonic genes in a zebrafish (ZF) ex vivo heart’s culture model by using Phenylephrine (PE) or a growth factors cocktail (GFs) as pro-hypertrophic/regeneration stimuli. Blebbistatin (BL) inhibitory action treatment was also studied for its involvement in blocking some of PE’s signal transduction actions. qRT-PCR and immunoblot analyses confirmed the upregulation of RACK1 in the PE- and GFs-treated groups. BL administration counteracted PE-induced hypertrophy and downregulated RACK1’s expression. Immunohistochemical analyses of the heart showed the co-localization of RACK1 and embryonic genes, namely, Gata4, Wt1, and Nfat2, under stimulation treatment, whereas resulted less expressed in the BL treatment. Cultured ZF heart cells activated via GFs treatment the enhanced expression of RACK1. Overexpression of RACK1 induced by transfection of recombinant RACK1 cDNA in ZF heart cells, enhances the expression of embryonic genes, especially after a week of GFs treatment. In summary, these results support the involvement of RACK1 in the induction of embryonic genes during cardiac hypertrophy/GFs stimulation in a fish heart model, which can be used as a mammal's alternative study model.
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Ceci et al. (2024) studied this question.
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