Evolution and applications of CRISPR-Cas9 technology in last decade

CRISPR-Cas9 technology has rapidly evolved and expanded its applications since its inception in 2015. This research paper provides a comprehensive overview of the advancements and applications of CRISPR-Cas9 technology from 2015 to 2023. We review key milestones in CRISPR-Cas9 research, including improvements in genome editing efficiency, novel applications in gene therapy and biotechnology, and emerging trends in CRISPR-based diagnostics and therapeutics. Additionally, we discuss the ethical considerations, regulatory challenges, and future prospects of CRISPR-Cas9 technology. Through a thorough analysis of the literature spanning nearly a decade, this paper aims to highlight the transformative impact of CRISPR-Cas9 technology on biomedical research and its potential for addressing pressing societal challenges.