One of the most frequently used biocatalysts for organic synthesis is lipase, a triacylglycerol hydrolase, that also catalyzes esterification in non‐aqueous solvents. Various non‐aqueous solvents such as organic solvents, ionic liquids, and pressurized carbon dioxide (CO 2 ) have been employed for lipase‐catalyzed reactions. Notably, pressurized CO 2 (supercritical CO 2 , liquid CO 2 , CO 2 ‐expanded liquid) serves as a functional green solvent. This review briefly outlines the properties of pressurized CO 2 relevant to biocatalysis and provides examples of utilizing pressurized CO 2 solvents for efficient synthesis with immobilized lipases.
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Matsuda et al. (2024) studied this question.
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