Supplementary Figure 2 from Prognostic Significance of SASP-Related Gene Signature of Radiation Therapy in Head and Neck Squamous Cell Carcinoma

Supplementary figure 2. Pathway analysis (A) Pathway analysis of SASP-related gene signature based on Protein–protein interaction network. The PPI confidence score lower than 0.9 and genes in the network with at least 5 degrees were respectively set as significant. Subsequently, the PPI network with scores> 5, node ≥ counts 5, node score = 0.2, and maximum depth = 100 as cut-off criteria. (B) Enrichment pathway of SASP-related gene signature based on GESA analysis. The human-ENSEMBLE-Gene-MsigDB chip was selected as the chip platform. FDR less than 0.05 after 1,000 random permutations were set as the cut-off criterion. (C) TNF-alpha signaling via NF-kappa B was enriched in cluster 1 of SASP-related gene signature (FDR <0.05) and G2M checkpoint enriched in cluster 2 of SASP-related gene signature (FDR <0.05) The enrichment score (y-axis) is shown. The top portion of the plot shows the ES (green line). (D) The ranked list of genes in TNF-alpha signaling via NF-kappa B of cluster1 (Right) and the ranked list of genes in the G2M checkpoint of cluster2 (Left) are shown.