Intracellular recording and dye filling of human myenteric neurons v1

This protocol explains how to make intracellular recordings and dye fills with glass micropipettes filled with potassium chloride and 5,6 carboxyfluorescein. It also includes immunohistochemical processing of dye fills. This protocol was used for the study: WP Yew, A Humenick, BN Chen, DA Wattchow, M Costa, PG Dinning, SJH Brookes (2023). Electrophysiological and morphological features of myenteric neurons of human colon revealed by intracellular recording and dye fills. Neurogastroenterology & Motility. e14538, 2023, https://doi.org/10.1111/nmo.14538)