Supplementary Figure S5 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer

Supplementary Figure S5. Expression of OPN in MC/9 cells and effect on T23 cell proliferation. A. Separate channels of immunofluorescence for DAPI (cyan), OPN (red) and WGA (blue) of MC/9, MC/9-CTR, MC/9-OPNf, and MC/9-iOPN cells showed in Fig. 2E. B. Quantification of A as percentage of OPN positive cells. C. Murine adenocarcinoma (T23) cells (50.000/well) were cultured either alone or with MC/9, MC/9-CTR, MC/9-OPNf or MC/9-iOPN cells (tumor cell:MC ratio 1:1). After 4 days the growth rate of cancer cells was evaluated through trypan blue count. Cancer cells and MC/9 could be distinguished thanks to their grown in adhesion or suspension, respectively. All histograms depict mean ± SD of biological replicates (represented by dots). One-way ANOVA followed by Tukey’s multiple comparison test was used: *, P < 0.05; **, P <0.01. Where P-value is not indicated, the comparison between groups is not statistically significant.