Supplementary Figure S7 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer

Supplementary Figure S7. Flow cytometry characterization of TNFa receptors and TNFa production. A. List of cytokines and chemokines tested through a multiplex immunoassay (Procarta-plex by Thermofisher) in the supernatants of ST4787 or T23 cells cultured either alone or in the presence of WT, OPN-/-, or MyD88-/- MCs. B.-C. Flow cytometry evaluation of TNFRs (CD120a and CD120b) on adenocarcinoma (T23 murine, 22Rv1 human) and NEPC (TC566 and TC411K murine, and LASCPC-01 human) cells. D. Gating strategy applied for intracellular detection of TNFa in MCs (CD49f-CD45+) by flow cytometry, in the co-cultures between MCs and tumor cells.