A Novel Antigen Design Strategy to Isolate Single‐Domain Antibodies that Target Human Nav1.7 and Reduce Pain in Animal Models

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Genetic studies have identified the voltage‐gated sodium channel 1.7 ( Na v 1.7 ) as pain target. Due to the ineffectiveness of small molecules and monoclonal antibodies as therapeutics for pain, single‐domain antibodies ( V H Hs ) are developed against the human Na v 1.7 ( hNa v 1.7 ) using a novel antigen presentation strategy. A 70 amino‐acid peptide from the hNa v 1.7 protein is identified as a target antigen. A recombinant version of this peptide is grafted into the complementarity determining region 3 ( CDR3 ) loop of an inert V H H in order to maintain the native 3D conformation of the peptide. This antigen is used to isolate one V H H able to i) bind hNa v 1.7, ii) slow the deactivation of hNa v 1.7, iii) reduce the ability of eliciting action potentials in nociceptors, and iv) reverse hyperalgesia in in vivo rat and mouse models. This V H H exhibits the potential to be developed as a therapeutic capable of suppressing pain. This novel antigen presentation strategy can be applied to develop biologics against other difficult targets such as ion channels, transporters and GPCRs.