Easy preparation of DNA Molecular Marker for a routine laboratory experiments.

During routine laboratory work in molecular biology, gel electrophoresis is used to follow experiments related to DNA, such as PCR, DNA extraction, and DNA digestion, and the use of molecular markers as important components for subsequent analysis is crucial, considering the quality of the DNA, the putative concentration, which is a qualitative measure of the molecular marker used as a reference, and the size of the DNA sample. There are nice and diverse molecular markers for use according to our needs; however, they could be expensive and inadequate for routine DNA analysis. In this work, we describe the preparation of a DNA molecular marker via a classical method. In essence, we digest Lambda phage DNA through two different restriction reactions, the StyI and HindIII enzymes, which produce the classical and popular DNA molecular marker used before the actual DNA Ladder molecular marker. We mix both digestion methods and produce a good and useful DNA molecular marker called Lambda HS. Both digestions produced 17 bands, from 23103 to 421 pb. The cost of production is also affordable for a typical molecular biology lab.