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In this study, we present an advanced system that integrates simultaneous pipetting and in-situ imaging using the Opentron OT-2 liquid handling robot. This system enables real-time monitoring and characterization of dynamic processes, such as hydrogel crosslinking, without any manual intervention. The platform’s modular design maintains cost-effectiveness and high-throughput capabilities while expanding the versatility of the OT-2 robot by incorporating imaging functionalities into the experimental workflow using a pick-and-place apparatus. Due to its modular architecture, based on an OT-2 robot, the system can be adapted for a wide range of laboratory applications at an affordable cost. This real-time imaging solution offers a practical approach to laboratory automation, leading to more efficient and data-driven experimentation. Although ionically crosslinked hydrogels were used as a proof-of-concept, this platform has potential applications across various materials systems, including crystallization dynamics, polymerization kinetics, and drug delivery system development.
Nazeri et al. (Wed,) studied this question.
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