Isolation of the Region Around Locus Coeruleus for Single Nucleus RNA Profiling v1

This protocol outlines the methodology for isolating the peri-locus coeruleus (peri-LC) region in mice for subsequent single nucleus RNA profiling. Key steps include the preparation of NMDG-aCSF buffer with precise pH and osmolarity, careful euthanasia and extraction of the brain, and the use of a vibratome to obtain 300 µm thick slices. The peri-LC region is identified and isolated using a biopsy puncher, followed by immediate shock-freezing in liquid nitrogen to preserve tissue integrity. This approach facilitates high-quality RNA profiling, essential for understanding the molecular characteristics of the locus coeruleus and its surrounding regions.