Efficient and precise targeting of the AAVS1 safe harbour locus in hPSCs. v2

Stably genetically-modified human pluripotent stem cells (hPSCs) are increasingly being used for studies relying on the consistent expression of the transgene of interest in human stem cells and their derivatives. Most often, the robustness of the transgene expression is achieved by its introduction into one of the members of an ever-expanding set of so-called "safe harbour"loci in the human genome. Here we describe a process of an efficient generation of high-quality hPSC clones with precise homology-directed targeting of the AAVS1(PPP1R2C) locus assisted by local DNA cutting using TALEN user-customisable nucleases.