Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection

A miniaturized electrospray interface consisting of a microfluidic nanosprayer and nanospray module is reported in the presented short communication. The nanosprayer was fabricated using silicon (Si) technology suitable for cost‐efficient high‐volume mass production. The nanospray module enabled the positioning of the nanosprayer in front of a mass spectrometry entrance and its coupling with capillary electrophoresis based on the liquid junction principle. A case study of top‐down and bottom‐up proteomic analyses of intact cytochrome c and its tryptic digest demonstrates the practical applicability of the developed interface.