Abstract Determination of total number of bacteria is vital for water pollution monitoring. However, fast, portable and quantitative on-site aquatic bacteria detection remains challenging. Here, a fluorescence sensor for bacterial concentration detection based on Ag@carbon dots composites (Ag@CDs) were developed. Fluorescence of CDs was quenched by plasmon-enhanced resonance energy transfer from CDs to nano Ag and recovered due to interaction with bacteria. Ag@CDs with controllable fluorescence “OFF-ON” was prepared through regulation of Ag and CDs composition reaction. Ag+ concentration-dependent fluorescence quenching and bacterial concentration-dependent fluorescence recovery provided Ag@CDs the support for bacterial quantitative detection. After exposure to Ag@CDs, bacteria quickly produced ROS which remained unchanged at 5 min, that is the basis of rapid test of bacteria. Ag@CDs-based fluorescence sensing system was optimized and exhibited a wide linear range of 7×104~4×107 CFU/mL, low detection limit of 4×104 CFU/mL, short response time of 5 min and high selectivity. The 96-well plate assay for quantitative measurement and test strips assay for semi-quantitation were developed for rapid in-field aquatic bacteria detection. Therefore, this study could give a new sight for application of Ag@CDs for rapid on-site aquatic bacteria detection in water quality monitoring.
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song et al. (2024) studied this question.
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