Tomogram Reconstruction and Gold Fiducial Removal with Warp, Etomo, and Fidder v1

While gold nanoparticle fiducials greatly improve tilt-series alignment in cryo-electron tomography data, they can cause artifacts during later image processing steps. We present a workflow for high-quality fiducial removal using fidder, an open-source python package. This protocol demonstrates how to flexibly incorporate fidder into tomogram reconstruction using Warp and Etomo.