Antagonism between JAK/STAT downstream targets controls stem cell proliferation, cell fate conversion and tumorigenesis

Proper proliferation and differentiation of adult stem cells maintains tissue homeostasis. However, how cell proliferation and fate conversion are regulated by niche signals remains poorly understood. Here, we systemically identify JAK/STAT downstream targets in adult Drosophila testis using multi-omics approaches. ubr5, encoding an HECT type E3 ligase, is identified as a putative JAK/STAT target. Depletion of ubr5 in somatic cyst cells affects the proliferation and differentiation of cyst stem cell (CySC) and germline stem cell (GSC). Importantly, ubr5-defective CySC-like cells adopt hub cell fate. Mechanistically, UBR5 interacts with Drumstick (Drm), another putative JAK/STAT target, through its UBR domain and mediates Drm poly-ubiquitination for proteolysis. Ectopic expression of drm mimics ubr5-depleted testes and further removal of drm significantly suppresses the defects observed in ubr5-depleted testes. Finally, the function of UBR5 in stem cell regulation is evolutionarily conserved. Collectively, antagonism between JAK/STAT targets controls JAK/STAT signaling duration, stem cell proliferation/differentiation, and cell fate conversion within the testicular niche. Thus our study uncovers the underlying mechanism of how stem cell proliferation and fate conversion are properly controlled during tissue homeostasis and tumorigenesis.