Figure 5 from Acetalax (Oxyphenisatin Acetate, NSC 59687) and Bisacodyl Cause Oncosis in Triple-Negative Breast Cancer Cell Lines by Poisoning the Ion Exchange Membrane Protein TRPM4

TRPM4 targeting and proteasomal degradation of TRPM4 by Acetalax and bisacodyl. A, Western blot of TRPM4 protein levels in MDA-MB468 following Acetalax treatment. B, Western blot of TRPM4 protein levels in MDA-MB468 following Acetalax and MG132 proteasome inhibitor treatment. C, Western blot of TRPM4 protein levels in MDA-MB468 following bisacodyl treatment. D, Western blot of TRPM4 protein levels in MDA-MB468 following bisacodyl and MG132 proteasome inhibitor treatment. The presence or absence of Acetalax or bisacodyl and MG132 is indicated in each experiment by + and/or − above each column. Cells were treated with Acetalax for 4 hours at 10 μmol/L and bisacodyl at 30 μmol/L. Cells were treated with MG132 for 4 hours at 20 μmol/L.