Figure 1 from mTORC1-Driven Protein Translation Correlates with Clinical Benefit of Capivasertib within a Genetically Preselected Cohort of PIK3CA-Altered Tumors

Statistical analysis of targeted and global proteome comparing protein expression in analyzed FFPE tumor tissues from the CB (green) and NCB (red) groups. A, Schematic of experimental design for phase I of the study. B, Results of targeted quantitation by iMALDI-MS. Boxplots of protein concentrations in the CB (green) vs. NCB (red) groups. Each point represents one patient tumor, averaged for multiple slides. P-values are given for a two-tailed t test. C, Heatmap of 50 selected proteins with highest differential expression between groups showing their normalized LFQ abundances across both analyzed batches. n = 23. Arbitrary identifiers are included for the purpose of identifying replicates from the same tumor. D, Volcano plot showing the fold change (log₂FC) of all protein expression features vs. P-value (−log₁₀P). E, PCA. Proximity of sample replicates originating from the same patients is indicated with a (dashed yellow line). F, Features ranked by VIP based on their contribution to the discrimination between the CB and NCB groups in the PLS-DA. The arrows on the right of the VIP Feature list show relative expression of that protein in the NCB group vs. the CB group.