Antimicrobial effect of (2-chloro-4-cyano-5-ethoxy-3,5-dioxopentan-2-yl) phosphonic acid against pathogenic bacteria and fungi

The synthesized organic compound ((2-chloro-4-cyano-5-ethoxy-3,5-dioxopentan-2-yl) phosphonic acid)) was evaluated against pathogenic microorganisms using agar well diffusion method. The results obtained reveled that, the diameters of inhibition zones were ranging between 24.0-36.3 mm for gram-negative bacteria, from 28.3 mm to 34.5 mm for gram-positive bacteria and between 18.2-20.3 mm for genus Candida. The MIC value 62.5 µg/mL showed that among gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa were the most sensitive to the compound compared to Acinetobacter baumannii and Klebsiella pneumoniae. Gram-positive bacteria (Staphylococcus aureus, Bacillus mesentericus and Bacillus subtilis) had the same sensitivity to the compound with 125 µg/mL as MIC value. Among fungal strains, Candida tropicalis with MIC value 62.5 µg/mL was the most sensitive to the compound compared to Candida albicans (250 µg/mL) and Candida guilliermondii (125 µg/mL).