Association of the Arg223Gln polymorphism of leptin receptor gene (LEPR) with clinical, laboratory, and instrumental data in asthma and diabetes type 2 comorbid patients

Aim. To study to the frequency of alleles and genotypes of the leptin receptor polymorphism (Arg223Gln) in comorbid patients with asthma and type 2 diabetes, as well as determine the association of leptin receptor genotypes with clinical, laboratory, and instrumental data. Materials and methods. Patients with asthma and type 2 diabetes were divided into three groups for comparative analysis, with 80 people in each group. All groups were subjected to the following procedures: clinical examination, determination of disease control using Asthma Control Test in patients with asthma, laboratory examination (determination of fasting blood glucose, glycated hemoglobin), spirometry, molecular genetic analysis of polymorphism of the leptin receptor (LEPR) Arg223Gln by PCR using kits for determining in the human genome. Nonparametric statistical methods were used to process the data obtained. Results. Patients with a combination of diabetes type 2 and asthma were found to have a higher incidence of G-allele polymorphism of the leptin gene (Arg223Gln) as compared to those with these diseases in isolation. The G-allele of the LEPR polymorphism (Arg223Gln) is associated with a higher incidence of diabetes type 2 in patients with asthma. The association of the G-allele in the LEPR gene was found in patients with a combination of asthma and diabetes type 2 with a failure to achieve the target value of fasting blood glucose, a lower parameter of respiratory function FEV1 and a lower control of asthma. Conclusion. The influence of the leptin receptor polymorphism on the combined course of asthma and type 2 diabetes mellitus is noted. An analysis of the leptin receptor polymorphism (Arg223Gln) is useful when identifying asthma patients at a higher risk of developing diabetes type 2.