Figure 3 from Human 3D Ovarian Cancer Models Reveal Malignant Cell–Intrinsic and –Extrinsic Factors That Influence CAR T-cell Activity

Primary omental fibroblasts induced CAR T-cell cytotoxicity against G164 cells in suspension spheroids. A and B, ELISA data showing IFNγ concentration after coculturing CAR T cells with OvCAR3 (A) and G164 (B) spheroids with and without FBs or FB-M for 2 days at 1:5 T:E ratio. Data plotted as mean ± SD for three CAR T-cell donors. Three different fibroblast donors were used for this experiment. Statistics performed using two-way ANOVA. C and D, Representative images (left) and quantification (right) of Incucyte killing assay in which OvCAR3 (C) and G164 (D) spheroids with and without FB or FB-M were treated with CAR T cells from one donor at 1:5 T:E ratio. Images shown are 3 days after treatment. Red, dead cells. Scale bars, 400 μm.