Effect of Direct Nicotinic Salts Laryngeal Exposure on the Physiologic Status of the Larynx

Background: The physiological effects of nicotine on the larynx are crucial for understanding its sensory impact. However, the lack of scientifically validated animal models to assess these effects has been a significant limitation in tobacco research. Objective: This study aimed to establish a novel animal model using advanced techniques to evaluate the direct physiological effects of nicotine and its salts on the larynx. Main Ideas: We utilized techniques such as oral buccal tube embedding, laryngeal electrode embedding, and real-time EMG signal recording. Mice were directly exposed to nicotine smoke, and changes in laryngeal EMG signals were dynamically monitored. The observations revealed a consistent increase in the amplitude of these signals with the duration of nicotine exposure, indicating a direct physiological effect of nicotine on the larynx. Furthermore, the effects of different nicotine salts on laryngeal physiology were objectively quantified by changes in the amplitude of electromyographic signals. Conclusion: Our study successfully established an animal model for assessing the physiological effects of direct nicotine exposure on the larynx. This model provides a valuable quantitative method for evaluating throat irritation and the sensory impact of nicotine salts, aiding in the development of safer and more satisfying tobacco products.