Zebrafish (Danio rerio) are a dynamic model organism, the larval stage (≤ 4 days post-fertilization (dpf)) of which offers a unique avenue for high-throughput in vivo investigation in a vertebrate that aligns strongly with the 3Rs of in vivo research. The light/dark locomotor assay is widely employed for studying larval behavior; however, there remains a lack of experimental consistency. Here, we provide step-by-step experimental andanalytical protocols for a standardized behavioral assay that exploits locomotion differences during light/dark phases. For details on the use and execution of this protocol, please refer to Hillman (2024).
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Hillman et al. (2024) studied this question.
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