Figure 2 from Exploration of Immune-Modulatory Effects of Amivantamab in Combination with Pembrolizumab in Lung and Head and Neck Squamous Cell Carcinoma

Multiplex IHC showing T cell subpopulations in the tumor microenvironment of HNSCC PDX (YHIM-3003) tumor after combination treatment of amivantamab and pembrolizumab. A, Whole slide images of tumor scan showing segmentation of tissues and cells. Helper CD4 and cytotoxic CD8 T cells were marked with FoxP3 (regulatory T cells) and GZMB (active cytotoxic T cells). Cancer cells were stained with Pan-CK. B, Stained whole slide images were quantified and presented in bar plots showing GZMB⁺ CD8 T cells and regulatory T cells in the tumor microenvironment, tumor nest and stroma in different treatment groups. Proportion of GZMB⁺ CD8 T cells in the total TME and the tumor nest was significantly increased in the combination group (P < 0.05). Each bar in the bar plot represents five mice that were internally sacrificed.