The following protocol is optimized for the detection of Salmonella Paratyphi A and Vibrio cholerae using multiplex qPCR assays from TNA extracted from environmental samples (wastewater samples). Total Nucleic Acid (TNA) extracted from the pellet particles is used as template in a real-time PCR assay which uses primer-probes specific for Salmonella Paratyphi A or Vibrio cholerae gene targets. Three different multiplex PCR assays were designed following standardization and validation of the primer probe combinations.
No takes yet. Share an insight, caveat, or question.
Abraham et al. (2024) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: