Multicolor fluorescence in situ hybridization and analysis v1

Antipsychotics are known to induce the expression of several immediate early genes (IEGs) via D2R antagonism, which then leads to changes in critical signaling pathways in iSPNs. To define the impact of LRRK2 kinase activity on the expression pattern ofIEGs in the iSPNs, we used multicolored single-molecule fluorescence in situ hybridization.