Pharmaceutical analysis is critical in ensuring the quality and safety of drug substances and formulations. High-performance thin-layer Chromatography (HPTLC) has emerged as a powerful analytical technique in the pharmaceutical industry due to its numerous advantages, including high separation efficiency, cost-effectiveness, and ease of sample preparation. One of its variants, Reversed-Phase High-Performance Thin-Layer Chromatography (RP-HPTLC), has gained immense popularity for analyzing nonpolar and slightly polar compounds, including drugs and their metabolites. This review paper draws attention to history and the recent developments in RP-HPTLC for pharmaceutical analysis. It highlights the advantages and limitations of RP-HPTLC, discussing its applications in drug analysis, impurity determination, stability-indicating assays, and more. In this study, recent advances in RP-HPTLC instrumentation and techniques were reviewed, including hyphenated methods, such as Reversed-Phase High-Performance Thin-Layer Chromatography coupled with Mass Spectrometry and Reversed-Phase High-Performance Thin-Layer Chromatography coupled with Nuclear Magnetic Resonance. Through this comprehensive analysis, the authors aim to underscore the potential of RP-HPTLC as a reliable and efficient analytical technique in the pharmaceutical industry and shed light on future trends in this field.
No takes yet. Share an insight, caveat, or question.
Pandya et al. (2024) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: