Knockdown of CSF1R molecules enhances the anti-tumor effects of CD8 + T lymphocytes in bladder cancer

Abstract Objective: In the study, we preliminary investigated the role of CSF1R molecules on CD8⁺ T cells in bladder cancer (BLCA) and its molecular mechanism. Methods: The effect of CSF1R on BLCA cell proliferation，migration and invasion was determined by cell-killing assay and transwell. In vivo experiments, tumor tissues were divided into four portions, one for histological staining, one for flow cytometry detection, one for mRNA gene sequencing analysis, and one for qPCR validation. Information related to 161 bladder cancer patients in Xiangya Hospital of Central South University in the past five years was collected and stained with relevant pathology. By calculating the Jordon index, CSF1R was included in the high expression group by expressing 0.39-fold or more in CD8⁺ T cells. Results: Knockdown of CSF1R molecule inhibited bladder cancer proliferation, migration, and invasion, and flow cytometry revealed that it could lead to increased infiltration of immune cells. mRNA sequencing and qPCR results suggested that Ckm was significantly decreased after CSF1R knockdown. Conclusion:Our study provides novel insights into the roles of CSF1R in BLCA progression and the underlying crosstalk of tumor metabolism and immune microenvironment.