Evaluation of the Western Blot Technique for the Detection of Lipopolysaccharides and Glycoproteins from Specific Brucella Antigens in Northeastern Mexico

Brucellosis is a zoonosis of worldwide distribution, and an economically important infection of humans and livestock.The main species have been isolated from human infections are B. abortus and B. melitensis.The objective was to evaluate the Western blot technique for the detection of lipopolysaccharides and glycoproteins from specific Brucella antigens in northeastern Mexico.The study was descriptive and cross-sectional where 79 Goats of Saneen, Alpina, Toggenburg and Nubia breeds were sampled from a herd of the Ejido La Victoria in Mapimí, Durango, as a test sieve rose bengal was processed, the extraction of total proteins was also carried out membrane of the blood samples and thus the detection of Brucella by Western-blot.Of the total of 79 goats sampled from the herd of Ejido La Victoria in the municipality of Mapimí, Durango, 34 were positive to the Rose Bengal test used as a screening test, resulting in a seroprevalence of 43,03% In the present work it was evident the lipopolysaccharide exposure of B. melitensis evidenced by the serological reaction observed with the Rose Bengal antigen and with respect to the Western blot the protein of 37 kDa of both samples was detected by the sera of the samples RB positive of B. melitensis.By the Western blot method, 28 immunodominant proteins were detected in sera evaluated previously with the Rose Bengal test, it was observed that from the group of 4 goat breeds studied, the exclusive presence of a protein in a specific breed was not found, giving understood that B. melitensis does not discriminate between races.It is proposed that the 37 kDa protein is the most suitable to be used as a good marker of B. melitensis in goats and can be taken as reference for further studies in order to establish specific biomarkers for the diagnosis of this etiological agent.