Supplementary Figures S1-S12 from The Pan-RAF–MEK Nondegrading Molecular Glue NST-628 Is a Potent and Brain-Penetrant Inhibitor of the RAS–MAPK Pathway with Activity across Diverse RAS- and RAF-Driven Cancers

Supplementary Figure S1 supports Figure 1 and contains cellular properties of NST-628 and a comparison with tramentib Supplementary Figure S2 supports Figure 2 and contains KSR1 data Supplementary Figure S3 supports Figure 2 and contains additional RAF-MEK crystal structures Supplementary Figure S4 supports Figure 2 and contains EGF stimulation experiment Supplementary Figure S5 supports Figure 2 and contains CRAF-MEK cryo-EM Supplementary Figure S6 supports Figure 3 and contains BRAF and MEK IPs, and CRAF IP/MS Supplementary Figure S7 supports Figure 4 and contains the full OMNI screen panel Supplementary Figure S8 supports Figure 4 and contains NST-628 RNAseq, signaling and proteomics studies Supplementary Figure S9 supports Figure 4 and contains NST-628 resistance studies Supplementary Figure S10 supports Figure 5 and contains HCT116 and IPC298 in vivo data Supplementary Figure S11 supports Figure 6 and contains NST-628 PK as well as SK-MEL-2 and MeWo in vivo data Supplementary Figure S12 Supports Figure 7 and contains KRAS G12C inhibitor combination studies