Supplementary Figures and Tables from Combining TIGIT Blockade with MDSC Inhibition Hinders Breast Cancer Bone Metastasis by Activating Antitumor Immunity

Supplementary Figure 1: Bone metastasis immunosuppressive microenvironment. Supplementary Figure 2: Gating strategy for RNA sequencing. Supplementary Figure 3: Differentially expressed genes during breast cancer bone metastasis progression. Supplementary Figure 4: Metastasis-infiltrating T cells are profoundly altered by metastatic progression. Supplementary Figure 5: Immune Checkpoint Receptors (ICRs) are expressed by metastasis-infiltrating T cells. Supplementary Figure 6: Targeting TIGIT results in activation of T cell mediated anti-tumor immunity. Supplementary Figure 7: (a) Spinal Cord Compression occurrence in treated mice. P-values were calculated using two-sided Chi square test. Supplementary Figure 8: TIGIT is highly expressed in human bone metastasis. Supplementary Table 1: Patient cohort data. Supplementary Table 2: Primers sequences.