P-333 Brucellosis Among Laboratory Workers: A Persistent Concern

Introduction In Tunisia, brucellosis is endemic in certain regions, especially in rural areas. It is an occupational disease, primarily affecting professionals in contact with infected animals and laboratory workers. The aim of this study was to describe the circumstances surrounding work- related accidents occurring in laboratory workers due to inhalation of Brucella. Methods A retrospective descriptive study involving three laboratory workers who consulted the occupational department in February 2023 to report work-related accidents following accidental inhalation of Brucella. Data collection was done from medical records. A visit to the microbiology laboratory was conducted. Results The study included two women and one man, aged 28, 37, and 42 years. They worked as physician, laboratory technician, and nurse. All three laboratory workers had handled positive blood culture for Brucella. Two laboratory workers remained asymptomatic, while one developed a sudorificalgic fever. The workplace investigation revealed the absence of hoods and respiratory masks. The patients were referred to the infectious diseases department, where chemoprophylaxis with Rifampicin and Doxycycline was prescribed for three weeks for the first patient. For all patients, serological tests (Wright and Rose Bengal serologies) were conducted after two weeks to one month, and the results were negative. The work-related accidents were reported. Discussion This study highlights the work-related accidents in laboratory workers due to the inhalation of Brucella and the importance of protective equipment in bacteriology laboratories. Post- exposure prophylaxis is discussed. Conclusion The development of clinical practice guidelines for employees exposed to Brucella is an essential tool for occupational health physicians.