A Study on Antimicrobial Activity of Endophytic Fungi from Garcinia mangostana L.

Mangosteen (Garcinia mangostana L) is a tropical fruit plant which is native to Asia, Australia, Africa and Polynesia which is endowed with potentially beneficial properties and so called as super-fruit.Mangosteen plant is widely used as antibacterial, antiviral, anti-fungal, anti-inflammatory, antioxidant, anti-allergic and anti-malarial agent, hence it posses those properties.It is an ideal source of endophytic fungi.Endophytes are the microorganisms which form symbiotic association with the host plant.They are often bacteria and fungi that within the plants without causing apparent harm to the host plant.Endophytic fungi have proven to be a source of secondary metabolites and several extracellular enzymes such as amylase, lipase, and protease.Fungal endophytes residing within these plants could also produce metabolites similar to or with more activity than that of their respective host do.These fungal enzymes as well as fungal biomass can be used for detoxification or in the bioremediation of industrial and agricultural waste and other polluting compounds.The production of enzymes from endophytic fungi for commercial use is an unexplored field.In the present work nine different types of endophytic fungi were isolated.Isolation and study of cultural characters of endophytic fungi were done using sabouraud dextrose agar, potato dextrose agar and malt extract agar and microscopic study has done using lactophenol cotton blue staining technique.By using Mueller Hinton agar medium antimicrobial activity checked, ENF1, ENF2, ENF3, ENF4 and ENF7 showed antimicrobial activity.