The Study of Changing Trend in Minimum Inhibitory Concentration (Mic) of Vancomycin Resistant Staphylococcus Aureus (Vrsa) by Agar Dilution Method and Its Comparison With E-Test

Background: The emergence of Vancomycin-Resistant Staphylococcus aureus (VRSA) among Methicillin-Resistant Staphylococcus aureus (MRSA) strains is a signicant concern in the medical industry. Vancomycin and other glycopeptide antibiotics are frequently used to treat infections caused by methicillin-resistant Staphylococcus aureus. In recent years, the incidence of vancomycin intermediate S. aureus (VISA) and vancomycin resistant S. aureus (VRSA) has increased in many parts of the world. Accurate calculation of the Minimum Inhibitory Concentration (MIC) is critical for conrming VRSA and directing suitable treatment. This study examines the accuracy and utility of two commonly used MIC measurement techniques: agar dilution and the E-test strip method. Aim And Objective: A comparison of the agar dilution method and the e-test strip method for determining the minimum inhibitory concentration (MIC) to conrm VRSA among MRSA. This was an observational cross-sectional study conducted in the De Material And Methods: partment of Microbiology at Government Medical College, Kota, Rajasthan.The samples were processed immediately on receiving the lab, if there was a delay, they were refrigerated at 40-70Celsius. A total of 185 clinical MRSA isolates were screened from 384 Staphylococcus aureus isolates, and vancomycin MICs were determined using both agar dilution and E-test strip procedures in accordance with CLSI guidelines. In the Results: current investigation, the Vancomycin screen agar method was used on 185 clinical isolates of MRSA, 6 (3.24%) of which were identied as VRSA. Out of the 185 MRSA isolates, two (1.08%) were VRSA and three (1.62%) were VISA. The incidence of VRSA was discovered to be 1.62%. Both the agar dilution and E-test strip methods identied 180 (97.30%) VSSAs. The MIC values of the 185 MRSA were determined using both the agar dilution method and the E-test strip method. In this work, the Agar dilution method was used as the gold standard for MIC determination. It produces exact and consistent results but is laborious and time-consuming. The e-test strip approach is a more convenient and faster option. It is simple to execute and analyse, however the ndings may vary compared to the agar dilution approach. Both the Conclusion: agar dilution method and the E-test strip approach are suitable for determining MIC values. Both the agar dilution and E-test strip methods provide slightly different MIC values. The e-test strip method is easier to use than the agar dilution method. The agar dilution method is time consuming and labor-intensive, yet it is a reliable gold standard approach for MIC determination. The optimum clinical management of VRSA infections requires the use of an appropriate MIC determination method.