Isolation, Characterization and Bioremediation Potential of Hydrocarbon Degrading Bacteria From Petroleum Contaminated Soil

The previous few decades have seen a surge in petroleum products.This pollution led to significant problems.Microorganisms are used in bioremediation process to clean petroleum contamination, it is able to break down petroleum hydrocarbons.The current research sought to identify the microorganisms found in oil-contaminated sites from two different geographic locations (Vadodara and Mandavi (Surat), Gujarat, India), to examine the development of isolated bacteria and their properties.The research used the enrichment culture technique to isolate bacteria.Bushnella-Hass (BH) broth supplemented with crude oil as a sole carbon source.Based on morphological and biochemical characterization, the isolates were identified as Bacillus spp.The isolates were morphologically identified as gram's negative, short rod shaped and red in colour.The growth of bacterial population showed by growth curve.Biodegradation assay were performed at lowest (1%) and highest (10%) concentration for 78 hrs by using selected bacterial culture.The ability of isolates to degrade oil was 52% done by