Spectrophotometric Determination of Sulfamethazole Drug by Using Azo Coupling Reaction

This research is based on one simple and good method for determining in their pure sta Sulfamethaxazone te and in pharmaceutical preparations. Spectrophotometric determination of using the azo co Sulfamethzole upling reaction in an aqueous solution and several standard solutions were prepared. The optimal conditions were also studied Effect of the type of acid and its volume, the influence of the base type and its volume, Effect of reagent volume (100 µg/ml), Effect of reaction time is color Product stability and effectiveness adding equence, Solvent effect, and Effect of temperature on the formation and the stability of colored product. The product for the Sulfamethzole drug is Orange at the wavelength of 489nm, the This method follows Beer's law in one respect of concentrations ranging from (1-12 µg/ml), and is the molar absorbance (39534.061 L/mol.cm). Sandal's sensitivity (0.0009 μg/cm), detection limit (0.3539 µg/ml), correlation coefficient value 0.999, and RSD% value 0.0010925, By applying the method to a pharmaceutical preparation containing Sulfamethzole , Sulfamethaxazone he recovery value ranged between 100.388075%.