Development of double haploid lines using anther culture method with different F2 combinations

Breeders are turning to rapid breeding methods in order to respond to the increasing population for the breeding of the wheat plant, which has a very important place in human nutrition. One of the biotechnological methods to shorten wheat breeding, which takes 15-20 years with classical breeding methods, is double haploid. The aim of this study is to obtain double haploid lines by taking wheat genotypes that have reached the F2 generation after hybridization into anther culture and thus shorten the breeding period. When looking at the response to anther culture of the 15 different F2 combinations used in the study, F2-7 and F2-10 genotypes stood out as the most successful genotypes. It was observed that 4 different groups were formed among 15 genotypes according to the success rate. It was concluded that the success of anther culture varies depending on the genotypes used, the nutrient media and the suitability of laboratory conditions.