Comparison of several salt water concentrations on salivary flora

Abstract Objective :Mouth washing involves soaking the mouth's mucosa, gums, and teeth in a solution. Its objectives include treating certain oral diseases and enhancing oral hygiene. Examining the impact of salt water mouthwashes on oral flora in vitro is the goal of this study. Methodology : in the Bacteriology Division of the Central Laboratory of Tishreen university Hospital, we carried out a cross-over clinical trial. From January 2021to July 2022, this study has a 6-month duration. We performed convenience sampling throughout this work. In our article, the same mouthwash was given to every participant at the same time, and the results were tracked throughout time (H1–H5). In order to prevent carry-over effects, a wash-out time of 3 days was observed between the interventions (mouthwashes with various saline solutions: 0.9%; 2%; 5.8% and 23%). To identify the bacterial species impacted by the mouthwashes, gram staining was done before and seven hours after the mouthwashes . We selected 10 subjects, from whom we collected 240 samples of saliva. There was a 1:1 sex ratio. Results :Our findings demonstrated that all solutions reduced the oral flora 2 minutes after rinsing (e.g., 5.8% saline reduced the original flora by half). The handmade saline (2%) exhibited an antibacterial impact on the oral flora that lasted for 3 hours, but the action of the 0.9% saline was only present for this period. However, only the 5.8% and 23% concentrations significantly reduced the oral flora as the sampling process went on. 5.8% salt content exhibited a 5-hour bactericidal effect, while 23% salt concentration had a 7-hour effect. We also observed a more pronounced action on gram positive bacteria. Conclusion: the 5.8% saline is more well-tolerated by the subjects and has long-lasting efficacy. Since the prepared saline in home has an antibacterial effect that lasts for three hours, it can be recommended for short-term purposes. Since the 23% saline has a strong, unpleasant taste, it should be avoided.