Antibacterials Effect of Tulsi (Ocimum sanctum) Powder Nanoparticle and its Scientific Evaluation by Employing Modern Scientific Tools

In recent years, nanotechnology has facilitated the development of Tulsi-based nanomedicine. This study explored the importance of Tulsi leaves nanopowder in traditional medicine and nanomedicine and its profound impact on the human body. Tulsi, also known as Holy Basil, has been recognized for its diverse therapeutic properties, including anti-inflammatory, antioxidant, and antimicrobial effects. Integrating traditional medicinal knowledge with cutting-edge nanotechnology has opened new avenues for advancing healthcare. Fresh Tulsi leaves were collected and ground using a mortar and pestle to obtain a coarse powder. The coarse powder was further ball milled for 25 hours in an ethanol medium using a high-energy ball mill method. XRD analysis revealed the crystal structure and the nanoparticle's estimated crystallite size was 21 nm. The average particle size was approximately 160 nm, calculated from the FE-SEM micrograph. The elements present in the Tulsi powder were estimated with the help of EDS. The antimicrobial activity of processed nanomedicine has enhanced due increase surface area of Tulsi nanoparticles and had a positive surface charge.