Synthetic Seed Production and Cryopreservation for Myrtle (Myrtus communis L.) Genotypes

This comprehensive study delves into synthetic seed production and cryopreservation for Myrtus communis L., presenting a novel approach to conserving plant genetic resources. Our research highlights shoot tips' encapsulation and subsequent conversion into plants following short-term (+4°C) and long-term (-196°C) preservation periods. The study rigorously compares the viability and efficiency of these cryopreserved seeds with conventional propagation methods. We extensively investigated various factors affecting the success of synthetic seed production, including explant selection, medium composition, and encapsulation techniques. Additionally, the impact of different preservation temperatures on synthetic seeds' viability and germination rates was a focal point. Our results demonstrate a significantly high conversion rate of synthetic seeds to plants post-cryopreservation, indicating the reliability of this method in germplasm conservation. The findings of this research are pivotal for conserving Myrtus communis L. genotypes and offer valuable insights and methodologies applicable to other plant species. This study marks a critical advancement in plant biotechnology and germplasm conservation, providing a sustainable solution for preserving valuable genetic resources in the face of escalating environmental challenges.