Phosphatase activity of phosphate solubilizing microbes as affected by organic P substrate and acidity

Phosphate solubilizing microbe in soil has capable to produce extracelluler enzyme, i.e. group of phosphatase enzyme which able to mineralized of organic P to inorganic P so that prepare high P for plant. The objective of this experiment was to examine the phosphatase activity from soil bacteria Pseudomonas mallei, Bacillus subtilis and fungi Aspergilus niger and Penicillium sp. in media containing different organic phosphorous and acidity. The result of experiment showed that the kind of organic phosphorous of medium affect phosphatase activity of Pseudomonas mallei, Bacillus subtilis, Aspergilus niger and Penicillium sp. In general, all microbe grown on medium with organic P substrate of phytic acid (myo-inositol hexakisphosphate) showed highest phosphatase activity compare to those grown on organic P substrate from glycerophosphate disodium salt, phenil phosphate or α-D-glucose 1-phosphate dosidium salt. Furthermore, the highest dissolve P was obtained from medium which contain glycerophosphate disodium salt.