Ultrasound-assisted Extraction of Β-glucan From Vietnam Pleurotus Citrinopileatus Enhances Anti-oxidant Activities

Introduction: Pleurotus citrinopileatus is a natural source of antioxidants and immune system support. Studies have shown that the glucan content in fresh Pleurotus citrinopileatus ranges from 10 to 40% by weight. Methods: β-glucan is extracted from Pleurotus citrinopileatus mainly by the hydrothermal method. In this study, β-glucan was collected from Pleurotus citrinopileatus using an ultrasound-assisted hydrothermal method. Beta-glucan from Vietnam Pleurotus citrinopileatus was evaluated through FTIR, UV-Vis, and SEM imaging. The optical absorption maximum of the extract was recorded at a wavelength of 257 nm. The infrared wave bands in the dried product were found to have characteristic functional groups of polysaccharide compounds. The morphology of β-glucan is a fiber with a diameter of 10÷15 um. Results: Bioassays of the antioxidant and immunogenicity of β-glucan from Pleurotus citrinopileatus using this technique are feasible. Conclusion: The study results show the potential of green and economic chemistry methods in extracting β-glucan from Pleurotus citrinopileatus.