Anticancer activity of pyoverdine (PVD) producing by antibiotic-resistant Pseudomonas aeruginosa isolated from burn and wound infections

Pseudomonas aeruginosa can cause diseases and multidrug resistance. It can produce many pigments, such as pyoverdine, which has anticancer properties. Cancer is still a major issue in medical science. Therefore, this study aimed to use highly efficient alternative treatments, such as pyoverdine. One hundred fifty samples of burns and wounds were collected from patients hospitalized in BabylIt hospitals. All the isolates were cultivated on various media to identify all specimens, including blood agar, MacConkey agar, and cetrimid agar. The isolates were tested for antibiotic susceptibility. P. aeruginosa was cultured in Luria-Bertani (LB) medium to stimulate its production of PDV. Congo red method and microtiter plate were used to determine biofilm production. The findings showed 50 isolates of P. aeruginosa were dispersed among patients, 35/50 (70%) burns and 15/50 (30%) wound infections and only four of the 50 isolates produced PVD. P. aeruginosa was examined against 17 common antibiotics(Aztreonam, Ceftazidime, Cefepime, Cefriaxone, Piperacillin_ tazobactam, Piperacillin, Gentamicin, Tobramycin, Netilmicin, Amikacin, Ciprofloxacin, Norfloxacin, Gatifloxacin, Levofloxacin, Imipenem, Doripenem, Meropenem)and the majority of isolates exhibited MDR. By using the Congo red method out of 50, 4(8%) isolates gave a positive ability to form biofilm as a qualitative method. Among 4 isolates, ps1 and ps4 were more productive, so it was tested to complete the study. PVD had anticancer activity against two types of cell lines: Lung cancer cells (A549) with inhibition range from 31.800in 400 µg/ml to 7.200 %in 25 µg/ml and skin cancer cells (A375) with cell vitality range from 55.600in 400 µg/ml to 8.533% in 25 µg/ml.