Figure S11 from HDAC6 Inhibition Releases HR23B to Activate Proteasomes, Expand the Tumor Immunopeptidome and Amplify T-cell Antimyeloma Activity

Fig. S11. Effect of the SGC-UBD253N (catalog number SML-3542, Sigma-Aldrich/Millipore-Sigma, Burlington, MA) is a negative control) probe on SIINFEKL-H2Kb presentation on E.G7-Ova cells (a) and effect of SGC-UBD253N on pan HLA-ABC presentation on three different MM cells (b). SGC-UBD253N is a closely related negative control for SGC-UBD253, a chemical probe for the HDAC6 UBD. E.G7-Ova (a) and MM cells (b) were treated with SGC-UBD253N at indicated concentration for 72h and the relative cell surface of the SIINFEKL-H2Kb complex detected by flow cytometry. Cells were then stained with a monoclonal antibody to SIINFEKL-H2Kb and quantitated using a BD-LSRII sorter interfaced with FlowJo software as above. MMCLs were treated with SGC-UBD253N at the indicated concentrations for 72 h. Cells were then stained with an anti- HLA-ABC (W6/32) antibody and quantitated using a BD-LSRII sorter interfaced with FlowJo software as above. Values represent the average of triplicate measurements. Error bars represent the SD.