CYToF has significantly advanced immunophenotyping, especially in exploratory settings where comprehensive characterization of immune populations is necessary but sample sizes are limited. It is a powerful tool for single-cell immunological assays, particularly for the detailed and simultaneous analysis of diverse immune cell populations. By using metal isotopes instead of fluorescent labels, mass cytometry eliminates the spectral overlap issues seen in optical flow cytometry, allowing robust analysis of around 60 individual parameters simultaneously. The technique involves increased complexity in the design, execution, and interpretation of experiments.
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Amouzgar et al. (2024) studied this question.
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