Key points are not available for this paper at this time.
This protocol provides detailed, step-by-step instructions for students and researchers to assemble transcriptomes from short reads generated by Illumina technology. In this tutorial, we will check the quality of the sequencing data and align the reads with a bacterial genome database to eliminate potential contamination. We will then use two approaches to assemble the transcripts: de novo assembly and alignment to the reference genome. The transcripts will be quantified and the assemblies evaluated.
Ferrari et al. (Wed,) studied this question.