Ghost imaging to see internal structure of a biological object with x-ray-based speckle illumination

We report an experimental demonstration of x-ray Ghost Imaging (GI) to observe the internal structure of a cardamom seed. To the best of our knowledge, this is the first use of GI to see the internal structure of biological samples. GI has been successfully demonstrated at visible and infrared wavelengths as a tool to perform precise imaging. At x-ray energies GI has a further potential to reach this goal with the additional advantage of reducing disruptive radiation doses to biological samples. The experiment was performed using 9.6keV x-rays at the 11-ID Coherent Hard X-ray Scattering (CHX) beamline of the National Synchrotron Light Source II (NSLS-II) facility at Brookhaven National Laboratory (BNL). This imaging technique has application potential in non-destructive examination of sensitive materials in the industrial and commercial sectors.