We conducted a multi-laboratory experiment across five labs to demonstrate reproducible microbiome formation, metabolite production, and plant growth using the EcoFAB 2.0 system. This protocol provided standardized instructions for the participating labs to implement the EcoFAB 2.0 system effectively, ensuring consistency in plant-microbiome experimentation. It includes annotated videos detailing each experimental step, utilizing a model bacterial community and the model grass Brachypodium distachyon in a hydroponic EcoFAB 2.0 setup.
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Novák et al. (2024) studied this question.
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