New eco-friendly spectrofluorometric approaches for the analysis of three antiepileptic drugs based on quenching of Acriflavine and silver nanoparticles

Two new spectrofluorometric methods have been developed for the quantitation of three antiepileptic drugs. The first method relied on monitoring the quenching effect of lamotrigine and carbamazepine on native fluorescence of acriflavine dye at 503nm (λex260 nm) in Britton Robinson buffer [pH 4]. The second one included the measuring of the quenching effect of carbamazepine and oxcarbazepine on the fluorescence intensity of silver nanoparticles at 531 nm (λex265 nm). Good linearity was obtained in method I over concentration range of 0.5 – 12 μg/mL ( r2 = 0.9999),with LOD of 0.16 and 0.157 and LOQ of 0.485 and 0.476 for lamotrigine and carbamazepine respectively. While in method II the linearity was in the range 1.0 -16 µg/mL ( r2 = 0.9998), with LOD of 0.167 and 0.259 and LOQ of 0.507 and 0.785 for carbamazepine and oxcarbazepine, respectively . The greenness of the methods was checked by three approaches; national environmental method index [NEMI], the analytical eco-scale and green analytical procedure index [GAPI].